Part:BBa_K4286205:Design

Snakin-1, a cysteine-rich basic antimicrobial peptide (AMP)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 73
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Evidence shows that the antimicrobial peptide Snakin-1 can effectively inhibit Rhizoctonia solani. In this project, we hope to express Snakin-1 constitutively in Trichoderma atroviride to improve its lethality against R. solani.

Source

Snakin-1 comes form Solanum bulbocastanum.

References

[1]Molla KA, Karmakar S, Molla J, Bajaj P, Varshney RK, Datta SK, Datta K. Understanding sheath blight resistance in rice: the road behind and the road ahead. Plant Biotechnol J. 2020 Apr;18(4):895-915. doi: 10.1111/pbi.13312. Epub 2020 Jan 29.

[2]Almasia NI, Bazzini AA, Hopp HE, Vazquez-Rovere C. Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Plant Pathol. 2008 May;9(3):329-38. doi: 10.1111/j.1364-3703.2008.00469.x.

[3]Kuddus MR, Yamano M, Rumi F, Kikukawa T, Demura M, Aizawa T. Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system. Biotechnol Prog. 2017 Nov;33(6):1520-1528. doi: 10.1002/btpr.2508. Epub 2017 Jun 12.